人Prolactin ELISA试剂盒(ab108679)

概述

  • 产品名称
    人Prolactin ELISA试剂盒
    参阅全部 Prolactin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Level 1 20 6.32 0.4 6.3%
    Level 2 20 19.22 1.19 6.18%
    Level 3 20 54.52 2.64 4.84%
    批次间
    样品 n Mean SD CV%
    Level 1 20 6.58 0.45 6.77%
    Level 2 20 21.34 1.3 6.11%
    Level 3 20 58.39 2.93 5.01%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.25 ng/ml
  • 范围
    5 ng/ml - 100 ng/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.


    Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.


    Get results in 90 minutes with Human Prolactin ELISA Kit (ab189570) from our SimpleStep ELISA® range.


     

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Anti-Prolactin-HRP conjugate 1 x 12ml
    Cover foil 1 unit
    Prolactin control 1 x 1ml
    Prolactin Standard 0: 0 ng/ml 1 x 1ml
    Prolactin Standard 1: 5 ng/ml 1 x 1ml
    Prolactin Standard 2: 10 ng/ml 1 x 1ml
    Prolactin Standard 3: 25 ng/ml 1 x 1ml
    Prolactin Standard 4: 50 ng/ml 1 x 1ml
    Prolactin Standard 5: 100 ng/ml 1 x 1ml
    Stop Solution 1 x 15ml
    Streptavidin Coated Microplate (12 x 8 wells) 12 Strips of 8 wells 1 unit
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 相关性
    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • 细胞定位
    Secreted
  • 别名
    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RATPRLSD1
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab108679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

实验方案

文献

ab108679 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam regarding ab108679.


I have confirmed that both antibodies in this kit are derived from Mouse.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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