人Prolactin ELISA试剂盒(ab108679)
Key features and details
- Sensitivity: 0.25 ng/ml
- Range: 5 ng/ml - 100 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Prolactin ELISA试剂盒
参阅全部 Prolactin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Level 1 20 6.32 0.4 6.3% Level 2 20 19.22 1.19 6.18% Level 3 20 54.52 2.64 4.84% 批次间 样品 n Mean SD CV% Level 1 20 6.58 0.45 6.77% Level 2 20 21.34 1.3 6.11% Level 3 20 58.39 2.93 5.01% -
样品类型
Serum -
检测类型
Sandwich (quantitative) -
灵敏度
0.25 ng/ml -
范围
5 ng/ml - 100 ng/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.
Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Prolactin-HRP conjugate 1 x 12ml Cover foil 1 unit Prolactin control 1 x 1ml Prolactin Standard 0: 0 ng/ml 1 x 1ml Prolactin Standard 1: 5 ng/ml 1 x 1ml Prolactin Standard 2: 10 ng/ml 1 x 1ml Prolactin Standard 3: 25 ng/ml 1 x 1ml Prolactin Standard 4: 50 ng/ml 1 x 1ml Prolactin Standard 5: 100 ng/ml 1 x 1ml Stop Solution 1 x 15ml Streptavidin Coated Microplate (12 x 8 wells) 12 Strips of 8 wells 1 unit Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
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相关性
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation. -
细胞定位
Secreted -
别名
- AV290867
- Lactogenic Hormone
- Luteotropic Hormone
see all -
数据库链接
- Entrez Gene: 5617 Human
- Omim: 176760 Human
- SwissProt: P01236 Human
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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