人PreAlbumin ELISA试剂盒(Transthyretin) (ab108895)

概述

  • 产品名称人PreAlbumin ELISA试剂盒(Transthyretin)
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.7%
    批次间
    样品 n Mean SD CV%
    Overall 9.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.08 ng/ml
  • 范围
    0.08 ng/ml - 31.25 ng/ml
  • 复检

    = 96 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PreAlbumin (Transthyretin) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PreAlbumin concentrations in plasma, serum, urine, saliva, milk and cell culture supernatants.

    A PreAlbumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PreAlbumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PreAlbumin captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 组织特异性Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • 疾病相关Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • 序列相似性Belongs to the transthyretin family.
  • 结构域Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • 细胞定位Secreted. Cytoplasm.
  • Information by UniProt
  • 别名
    • Amyloid polyneuropathy
    • Amyloidosis I
    • ATTR
    • Carpal tunnel syndrome 1
    • CTS
    • CTS1
    • Dysprealbuminemic euthyroidal hyperthyroxinemia
    • Dystransthyretinemic hyperthyroxinemia
    • Epididymis luminal protein 111
    • HEL111
    • HsT2651
    • PALB
    • Prealbumin
    • Prealbumin amyloidosis type I
    • Prealbumin Thyroxine-binding
    • Senile systemic amyloidosis
    • TBPA
    • Thyroxine binding prealbumin
    • Transthyretin
    • TTHY_HUMAN
    • TTR
    • TTR protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human PreAlbumin ELISA Kit (Transthyretin) 图像

  • Standard curve of Hu PreAlbumin, with background signal subtracted (duplicates; +/- SD).
  • PreAlbumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • PreAlbumin detected in cell supernatants, results shown after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108895.

实验方案

Human PreAlbumin ELISA Kit (Transthyretin) (ab108895)参考文献

ab108895 has not yet been referenced specifically in any publications.

Product Wall

The 10x diluent can have usual particulate that forms. These are simple salt deposits that the process of diluting breaks up and dissolves and there is no worry that this will affect results from the kit as you have also seen in your experiments.
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You should use the protocol supplied in the box with the kit as the method is optimized for that batch of kit components.
In this case the difference relates mainly to extra information supplied with lot 123855 and minor differences in incubation ...

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Our source for this assay claims "the kit will measure total prealbumin level" and that "it can recognize all forms of prealbumin".

Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding your enquiry.

This kit has been tested with samples from many different species, including bovine, monkey, mouse...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"