Anti-Human Polyoma virus JCV capsid protein VP1抗体[8E8] (ab34756)


  • 产品名称
    Anti-Human Polyoma virus JCV capsid protein VP1抗体[8E8]
  • 描述
    小鼠单克隆抗体[8E8] to人Polyoma virus JCV capsid protein VP1
  • 特异性
    Does not react with measles and mumps nucleocapsid proteins.
  • 经测试应用
    适用于: Indirect ELISA, WB, ICC/IFmore details
  • 种属反应性
    Reacts with major capsid proteins VP1 of hamster polyomavirus (HaPyV) and SV40. It does not cross-react with major capsid protein VP1 human polyomavirus BKV and murine polyomavirus (MuPyV)
  • 免疫原

    Recombinant full length protein corresponding to Human Polyoma virus JCV capsid protein VP1. Recombinant full length purified major capsid protein VP1 of human polyomavirus JCV expressed in yeast S.cerevisiae.


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
  • 克隆编号
  • 骨髓瘤
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab34756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.
ICC/IF Use at an assay dependent concentration. PubMed: 25155602


  • 相关性
    The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.

Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] 图像

  • Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 µg/mL.

  • ab34756 staining Human Polyoma virus JCV capsid protein VP1 in human fetal glial cell (SVG-A) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + BSA) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

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Anti-Human Polyoma virus JCV capsid protein VP1 antibody [8E8] (ab34756)参考文献

This product has been referenced in:
  • Sharma BN  et al. Antiviral effects of artesunate on JC polyomavirus replication in COS-7 cells. Antimicrob Agents Chemother 58:6724-34 (2014). ICC/IF . Read more (PubMed: 25155602) »

See 1 Publication for this product

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We unfortunately do not sell this product, Human Polyoma virus JCV. This product however might be available from different companies. Please check it online. You can also try searching in bio compare websi...

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Immunocytochemistry/ Immunofluorescence
Human Cell (Fetal glial cell (SVG-A))
Fetal glial cell (SVG-A)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C

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Verified customer

提交于 Jul 24 2015

Die Spezifitätsnotiz beschreibt folgendes:

ab34756 bindet das Major Capsidprotein VP1 von humanem Polyoma Virus JCV und das Capsidprotein VP1 von hamster polyomavirus (HaPyV) and Simian Virus 40 (SV40).

ab34756 kreuz-...

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ab34756 recognizes the C-terminal part of human polyomavirus JCV capsid protein VP1. The epitope is localized between 290 and 384 aa residues of JCV capsid protein VP1.

DISCOUNT CODE: ***********
Expiration date: **************
I am very pleased to hear you would like to accept our offer and test ab34756 in IHC. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, ...

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Thank you very much for your interest in ab34756.
To our knowledge, ab34756 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab34756 now, test it in IHC and submit feedback to us in the form of an Abre...

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Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this...

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Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal...

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DISCOUNT CODE: ****** Expiration date: February 11, 2012 I am very pleased to hear you would like to accept our offer and test ab34756 in ICC/IF. This code will give you one free primary antibody before the expiration date. To redeem this offer...

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