人Platelet Glycoprotein IIb + IIIa ELISA试剂盒(ab108851)

概述

  • 产品名称
    人Platelet Glycoprotein IIb + IIIa ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.4%
    批次间
    样品 n Mean SD CV%
    Overall 8.2%
  • 样品类型
    Plasma, Platelets, Cell Lysate
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.5 ng/ml
  • 范围
    1.25 ng/ml - 40 ng/ml
  • 复检

    101 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelets, platelet-rich plasma and cell culture lysate.

    A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Platelet Glycoprotein IIb + IIIa ELISA Kit 图像

  • Representative Standard Curve using ab108851.

实验方案

Human Platelet Glycoprotein IIb + IIIa ELISA Kit (ab108851)参考文献

This product has been referenced in:
  • Graf ME  et al. Biological reproducibility of circulating P-Selectin, Thrombopoietin, GPIIb/IIIa and Thrombomodulin over one year. Clin Biochem N/A:N/A (2017). ELISA ; Human . Read more (PubMed: 28571971) »

See 1 Publication for this product

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Thank you for your enquiry and interest in our products. I am very sorry for my delayed response.

I can confirm that the proper value for GPIIbIIIA in human plasma is about 600 ng/ml.

This kit will allow 1 x 96 tests and the price i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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