人Plasminogen ELISA试剂盒(PLG) (ab108893)

概述

  • 产品名称人Plasminogen ELISA试剂盒(PLG)
    参阅全部 Plasminogen 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.2%
    批次间
    样品 n Mean SD CV%
    Overall 7.5%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 1 ng/ml
  • 范围
    2.5 ng/ml - 30 ng/ml
  • 复检

    = 101 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Plasminogen (PLG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Plasminogen concentrations in Human plasma, serum, urine, saliva, milk, tissue samples, cerebrospinal fluid and cell culture supernatants.


    A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.


    For higher sensitivity in only 90 minutes, we recommend Human Plasminogen ELISA Kit (ab196262) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • 组织特异性Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • 疾病相关Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.
  • 序列相似性Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • 结构域Kringle domains mediate interaction with CSPG4.
  • 翻译后修饰N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • 细胞定位Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt
  • 别名
    • Plasmin
    • Plasmin light chain B
    • PLG
    • PLMN_HUMAN
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Plasminogen ELISA Kit (PLG) 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Plasminogen measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum and plasma samples diluted 20000-160000 fold. Other samples diluted 1-100 fold.

实验方案

Human Plasminogen ELISA Kit (PLG) (ab108893)参考文献

This product has been referenced in:
  • Banville N  et al. A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM). PLoS One 9:e105365 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25133674) »

See 1 Publication for this product

Product Wall

Abreviews
Review of Abcam Plasminogen Human ELISA Kit:

The Plasminogen ELISA, using the Abcam human ELISA kit Ab108893, Lot GR270028-6, was performed by an experienced laboratory technician at an accredited university. The goal of the experiment was to determine the cross-reactivity for rhesus macaque in both EDTA prepared plasma and in serum samples.

Overall, the kit, like other Abcam ELISA kits, was easy to follow and simple to use.

Our experiment consisted of using EDTA-prepared plasma samples and serum samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Samples were diluted serially in a range from 1:5000 to 1:80000 in the given diluent in duplicate. The rest of the assay was performed per protocol’s instructions.

At 1:80000, all samples were below the range of detection. At 1:40000, most samples were below the range of detection. At 1:20000 a few samples were below the range of detection, but most were in range. 1:5000 and 1:10000 were both in the range of detection for all sample types *subtracted ODs in the 0.6 and 0.5 range respectively). The data presented are the extrapolated values.

Overall, the kit is cross-reactive with both Rhesus plasma and Serum, however we were unable to confirm linearity of detection and value ranges are less than expected.
Username

Abcam user community

Verified customer

提交于 Jun 18 2016

Abreviews
Used the kit for the first time and diluted my plasma samples (citrate plasma) 1:20000 as suggested in the manual.
This dilution was right for almost all of my samples.
Followed the kit's instructions and had my results in about 4h.

Will use it again and can highly recommend it.
Username

Abcam user community

Verified customer

提交于 Aug 21 2014

The kit will recognize the plasmin A chain. This information is according to the uniprot information given below:

Plasmin heavy chain A: aa 20-580
Plasmin heavy chain A, short form: aa 98-580
Plasmin light chain B: 581-810

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Thank you for contacting us and your interest in our products.

Please let me reassure you that the Plasminogen Human ELISA Kit (ab108893) is suitable for use with serum samples. I am sorry for any confusion caused but this discrepancy, we will...

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Thank you for contacting us.

We do have a Plasminogen Human ELISA kit as ab108893, but this has not been tested for cross-reactivity with angiostatin.

http://www.abcam.com/Plasminogen-Human-ELISA-Kit-ab108893.html

We do h...

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Thanks for your enquiry!

Please contact our bulk order specialist, Andrea Gray at mailto:Andrea.Gray@abcam.com, regarding discounts on bulk order purchases.

I hope this is helpful. Please contact me again if you have any further que...

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Thank you for your reply.

I am happy to confirm that we offer an ELISA kit for human plasminogen:

ab108893 (Plasminogen Human ELISA Kit)

http://www.abcam.com/index.html?datasheet=108893 (or use the following: http://www.ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"