Anti-Human placental lactogen (epitope 2)抗体[INN-hPL-37] (ab11396)

概述

  • 产品名称
    Anti-Human placental lactogen (epitope 2)抗体[INN-hPL-37]
  • 描述
    小鼠单克隆抗体[INN-hPL-37] to人placental lactogen (epitope 2)
  • 特异性
    Radioimmunological direct binding studies were performed with 125I-labelled. hPL, hGH, hPRL and increasing amounts of INN-hPL-37. In addition, competitive RIA with unlabelled hGH, hPRL and hPL were used for characterization. In competitive RIA cross reactivity with hGH and hPRL was below 1%. Direct binding demonstrated 39% reactivity with hGH but no cross reactivity with hPRL.
  • 经测试应用
    适用于: RIAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human placental lactogen.

  • 常规说明
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide.
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purification by affinity chromatography.
  • 克隆
    单克隆
  • 克隆编号
    INN-hPL-37
  • 骨髓瘤
    x63-Ag8.653
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA Use at an assay dependent dilution.

靶标

  • 相关性
    Placental lactogen is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • choriomammotropin antibody
    • Chorionic somatomammotropin A antibody
    • Chorionic somatomammotropin hormone 1 (placental lactogen) antibody
    • chorionic somatomammotropin hormone 1 antibody
    • Chorionic somatomammotropin hormone 2 antibody
    • CS 1 antibody
    • CS-2 antibody
    • CS-B antibody
    • CSA antibody
    • CSB antibody
    • CSH1 antibody
    • CSMT antibody
    • hCSB antibody
    • Lactogen precursor antibody
    • Lactogen, placental antibody
    • PL antibody
    • placental lactogen antibody
    see all

Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37] 图像

  • ab11396 at 1/100 staining human BeWo cells (placenta, choriocarcinoma) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary antibody. The image is shown in the upper right hand panel. In this experiment ab15554 was used as a positive control (bottom left hand panel). Cells were counter stained with DAPI (upper left hand panel).

    See Abreview

Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)参考文献

ab11396 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Human recombinant hPL)
Loading amount
0.1 µg
Specification
Human recombinant hPL
Blocking step
Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Stoil Dmitrov

Verified customer

提交于 Apr 16 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (BeWo, placenta, choriocarcinoma)
Specification
BeWo, placenta, choriocarcinoma
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Stoil Dmitrov

Verified customer

提交于 Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册