Radioimmunological direct binding studies were performed with 125I-labelled. hPL, hGH, hPRL and increasing amounts of INN-hPL-37. In addition, competitive RIA with unlabelled hGH, hPRL and hPL were used for characterization. In competitive RIA cross reactivity with hGH and hPRL was below 1%. Direct binding demonstrated 39% reactivity with hGH but no cross reactivity with hPRL.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Placental lactogen is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites.
Immunocytochemistry/ Immunofluorescence - Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
ab11396 at 1/100 staining human BeWo cells (placenta, choriocarcinoma) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary antibody. The image is shown in the upper right hand panel. In this experiment ab15554 was used as a positive control (bottom left hand panel). Cells were counter stained with DAPI (upper left hand panel).