人PF4 ELISA试剂盒(CXCL4) (ab100628)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.58 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人PF4 ELISA试剂盒(CXCL4)
参阅全部 PF4 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
20.58 pg/ml - 15000 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 108.2 101% - 116% Serum 122.4 110% - 130% Plasma 119.6 111% - 127% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s PF4 (CXCL4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PF4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human PF4 ELISA Kit (CXCL4) (ab189573) from our SimpleStep ELISA® range.
-
说明
Optimization may be required with urine samples.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human PF4 2 units PF4 Microplate (12 x 8 wells) 1 unit Recombinant Human PF4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
Binds non-covalently to a proteoglycan molecule. -
细胞定位
Secreted. - Information by UniProt
-
别名
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
see all -
数据库链接
- Entrez Gene: 5196 Human
- Omim: 173460 Human
- SwissProt: P02776 Human
- Unigene: 81564 Human
相关产品
-
SimpleStep ELISA kits
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab100628 被引用在 4 文献中.
- Del Amo C et al. Unraveling the Signaling Secretome of Platelet-Rich Plasma: Towards a Better Understanding of Its Therapeutic Potential in Knee Osteoarthritis. J Clin Med 11:N/A (2022). PubMed: 35159924
- Del Amo C et al. Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings. Biomedicines 9:N/A (2021). PubMed: 34440227
- Dai B et al. lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-ß pathway. Oncol Lett 18:5923-5929 (2019). PubMed: 31788066
- Yang J et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). PubMed: 29754444