人Perforin酶联免疫吸附(ELISA)套装(without plates) (ab83709)

概述

  • 产品名称人Perforin酶联免疫吸附(ELISA)套装(without plates)
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Other biological fluids
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 40 pg/ml
  • 范围
    62.5 pg/ml - 2000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Perforin Human in vitro ELISA Set is designed for the quantitative measurement of Perforin in cell culture supernatants and buffered solutions.

     

    The Perforin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step. This ELISA set is intended to measure Perforin in cell -culture supernatants, as well as in serum samples*. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    *Special note for serum & plasma quantification: high and non linear detection level is found in human serum and plasma samples. The high signal appears not dependant to the antibody pairs but probably to the matrix and/ or perforin interaction with other molecules. Consequently serum and plasma quantification is under the scientist responsibility and specific investigations. Remark: no detection signal was found in fetal calf serum, rat, mouse and horse serum

     

    Sensitivity: < 40 pg/ml

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents

性能

  • 相关性Perforin is one of the major cytolytic proteins of cytolytic granules. One of the main pathways of lymphocyte mediated cytolysis entails the secretion onto target membranes of cytolytic granules contained in cytolytic effector lymphocytes of T cell or NK cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin is involved in the killing mediated by cytotoxic lymphocytes, which represents an important mechanism in the immune defense against tumors and virus infections. Human perforin is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecular weight of 70 to 75 kDa. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9 and homology between perforin and C9 has been demonstrated at their respective functionally conserved regions. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested.
  • 细胞定位Cell Membrane and Cytoplasmic
  • 别名
    • Cytolysin
    • FLH2
    • HPLH2
    • P1
    • Perforin 1
    • PFP
    • Pore forming protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab83709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Perforin ELISA Set (without plates) 图像

  • Perforin Standard curve ranging from 62.5 to 2000 pg/mL

Human Perforin ELISA Set (without plates) (ab83709)参考文献

ab83709 has not yet been referenced specifically in any publications.

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