人PDGF Receptor beta ELISA试剂盒(ab100626)

概述

  • 产品名称
    人PDGF Receptor beta ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 90.39 76% - 104%
    Serum 109.6 97% - 118%
    Plasma 96.23 81% - 117%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PDGF Receptor beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF Receptor beta in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF Receptor beta coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF Receptor beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF Receptor beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF Receptor beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100626.

实验方案

文献

ab100626 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting Abcam.

Please find attached the Certificate of Compliances for ab100624 and ab100626.

If there is anything else I can help you with, please do not hesitate to contact me.

Thank you for contacting Abcam. Attached, please find the Certificates of Compliance you requested. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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