人PDGF Receptor beta ELISA试剂盒(ab100626)

概述

  • 产品名称人PDGF Receptor beta ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 90.39 76% - 104%
    Serum 109.6 97% - 118%
    Plasma 96.23 81% - 117%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PDGF Receptor beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF Receptor beta in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF Receptor beta coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF Receptor beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF Receptor beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF Receptor beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • 组织特异性Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • 疾病相关Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • 别名
    • Beta type platelet derived growth factor receptor
    • CD140 antigen like family member B
    • CD140b
    • JTK12
    • PDGF R beta
    • PDGF subunit B
    • PDGF-2
    • Pdgfb
    • PDGFB_HUMAN
    • PDGFR
    • PDGFR beta
    • PDGFR1
    • PDGFRB
    • Platelet derived growth factor receptor beta
    • Platelet derived growth factor receptor beta polypeptide
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto-oncogene c-Sis
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human PDGF Receptor beta ELISA Kit 图像

  • Representative Standard Curve using ab100626.

实验方案

Human PDGF Receptor beta ELISA Kit (ab100626)参考文献

ab100626 has not yet been referenced specifically in any publications.

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