人PDGF Receptor beta ELISA试剂盒(ab100626)

概述

  • 产品名称人PDGF Receptor beta ELISA试剂盒
  • 检测方法Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    24.69 pg/ml - 18000 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 90.39 76% - 104%
    Serum 109.6 97% - 118%
    Plasma 96.23 81% - 117%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PDGF Receptor beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF Receptor beta in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF Receptor beta coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF Receptor beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF Receptor beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF Receptor beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • 疾病相关Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • Beta platelet derived growth factor receptor
    • Beta-type platelet-derived growth factor receptor
    • CD 140B
    • CD140 antigen-like family member B
    • CD140b
    • CD140b antigen
    • IBGC4
    • IMF1
    • JTK12
    • OTTHUMP00000160528
    • PDGF R beta
    • PDGF-R-beta
    • PDGFR
    • PDGFR 1
    • PDGFR beta
    • PDGFR1
    • PDGFRB
    • PGFRB_HUMAN
    • Platelet derived growth factor receptor 1
    • Platelet derived growth factor receptor beta
    • Platelet derived growth factor receptor beta polypeptide
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human PDGF Receptor beta ELISA Kit 图像

  • Representative Standard Curve using ab100626.

实验方案

Human PDGF Receptor beta ELISA Kit (ab100626)参考文献

ab100626 has not yet been referenced specifically in any publications.

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