人PDGF BB ELISA试剂盒(ab100625)

概述

  • 产品名称人PDGF BB ELISA试剂盒
    参阅全部 PDGF BB 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    0.549 pg/ml - 400 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 94.37 83% - 105%
    Tissue Extracts 92.78 82% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF BB in cell lysates and tissue lysates.


    This assay employs an antibody specific for Human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human PDGF BB ELISA Kit (ab184860) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • 组织特异性Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • 疾病相关Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • 别名
    • Becaplermin
    • c sis
    • FLJ12858
    • Oncogene SIS
    • PDG B chain
    • PDGB
    • PDGF 2
    • PDGF B
    • PDGF B chain
    • PDGF subunit B
    • PDGF-2
    • PDGF2
    • Pdgfb
    • PDGFB/COL1A1 fusion gene
    • PDGFB_HUMAN
    • Platelet derived growth factor 2
    • Platelet derived growth factor B chain
    • Platelet derived growth factor beta polypeptide
    • Platelet derived growth factor beta polypeptide (oncogene SIS)
    • Platelet derived growth factor subunit B
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto oncogene c Sis
    • Proto-oncogene c-Sis
    • Simian sarcoma viral (v sis) oncogene homolog
    • SIS
    • SSV
    • V sis platelet derived growth factor beta polypeptide
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab100625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human PDGF BB ELISA Kit 图像

  • Representative Standard Curve using ab100625.

实验方案

Human PDGF BB ELISA Kit (ab100625)参考文献

ab100625 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been test...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"