人PDGF AB ELISA试剂盒(ab100623)

概述

  • 产品名称人PDGF AB ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    10.24 pg/ml - 2500 pg/ml
  • 复检

    74 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 74.29 68% - 78%
    Serum 73.96 67% - 78%
    Plasma 74.85 67% - 79%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PDGF AB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF AB coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.
  • 别名
    • PDGF A chain
    • PDGF B chain
    • PDGF1
    • PDGF2
    • PDGFA
    • PDGFB
    • Platelet derived growth factor AB heterodimer
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human PDGF AB ELISA Kit 图像

  • Representative Standard Curve using ab100623.

  • Representative Standard Curve using ab100623.

实验方案

Human PDGF AB ELISA Kit (ab100623)参考文献

ab100623 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

I can confirm that the capture antibody of ab100623 binds the PDGF-B subunit.

The detection antibody binds the PDGF-A subunit but only in the heterodimeric form (ie. PDGF-AB).

The detection anti...

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Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"