人PDGF AA ELISA试剂盒(ab100622)

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概述

  • 产品名称
    人PDGF AA ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 40 pg/ml
  • 范围
    40 pg/ml - 30000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 92.54 82% - 102%
    Serum 76.71 70% - 83%
    Plasma 78.06 72% - 85%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Human PDGF AA (lyophilized) 2 vials
    PDGF AA Microplate (12 x 8 wells) 1 unit
    Recombinant Human PDGF AA Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.
  • 序列相似性
    Belongs to the PDGF/VEGF growth factor family.
  • 结构域
    The long form contains a basic insert which acts as a cell retention signal.
  • 细胞定位
    Secreted. Released by platelets upon wounding.
  • Information by UniProt
  • 别名
    • PDGF 1
    • PDGF A
    • PDGF A chain
    • PDGF subunit A
    • PDGF-1
    • PDGF1
    • PDGFA
    • PDGFA_HUMAN
    • Platelet derived growth factor A chain
    • Platelet derived growth factor alpha chain
    • Platelet derived growth factor alpha polypeptide
    • Platelet derived growth factor subunit A
    • Platelet-derived growth factor A chain
    • Platelet-derived growth factor alpha polypeptide
    • Platelet-derived growth factor subunit A
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab100622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100622.

    实验方案

    文献

    ab100622 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab100622？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    2 reviews or Q&A

    10
    Votes: Highest First

    Dear technical team,

    Our company has order a PDGF ELISA (ab100622) Kit from abcam. We would like to have a positive control of human (and murine) PDGF-A. Do you know where we can find one of them?

    Regards,

    Thank for your enquiry and interest.





    I would like to confirm the this kit contains 2 vials of Standards: recombinant human PDGF-AA





    However, currently we also have we have 4 products PDGF...

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    Dear technical team,   Our customer would like to purchase PDGF ELISA Kit from abcam.   http://www.abcam.com/index.html?pageconfig=searchresults&pt=4&search=pdgf   I have searched 5 products and the customer's sample is cell culture s...

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    Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been test...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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