人PDGF AA ELISA试剂盒(ab100622)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 40 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PDGF AA ELISA试剂盒
参阅全部 PDGF AA 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 40 pg/ml -
范围
40 pg/ml - 30000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 92.54 82% - 102% Serum 76.71 70% - 83% Plasma 78.06 72% - 85% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-Human PDGF AA 2 vials PDGF AA Microplate (12 x 8 wells) 1 unit Recombinant Human PDGF AA Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
结构域
The long form contains a basic insert which acts as a cell retention signal. -
细胞定位
Secreted. - Information by UniProt
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别名
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
数据库链接
- Entrez Gene: 5154 Human
- Omim: 173430 Human
- SwissProt: P04085 Human
- Unigene: 535898 Human
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab100622 被引用在 3 文献中.
- Chambliss C et al. Elevated neuregulin-1β levels correlate with plasma biomarkers of cerebral injury and high stroke risk in children with sickle cell anemia. Endocr Metab Sci 3:N/A (2021). PubMed: 35935682
- Dai Q et al. Modulation of Human Neutrophil Peptides on P. aeruginosa Killing, Epithelial Cell Inflammation and Mesenchymal Stromal Cell Secretome Profiles. J Inflamm Res 12:335-343 (2019). PubMed: 31908518
- Wood MD et al. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro. Sci Rep 8:5805 (2018). PubMed: 29643433