Anti-Human Papillomavirus 16 (E7)抗体[289-17013] (ab30731)

概述

  • 产品名称Anti-Human Papillomavirus 16 (E7)抗体[289-17013]
    参阅全部 Human Papillomavirus 16 (E7) 一抗
  • 描述
    小鼠单克隆抗体[289-17013] to人Papillomavirus 16 (E7)
  • 经测试应用适用于: IP, WB, ELISA, RIA, Flow Cyt, IHC-Frmore details
  • 免疫原

    Full length protein (Human) -HPV 16 E7 synthesized in S. pombe.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • 纯度>90% by SDS-PAGE
  • 纯化说明Protein A chromatography.
  • 克隆单克隆
  • 克隆编号289-17013
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab30731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription
  • 细胞定位Nuclear
  • 别名
    • E7 antibody
    • HPV16 E7 antibody
    • Protein E7 antibody
    • Transforming protein antibody

Anti-Human Papillomavirus 16 (E7) antibody [289-17013] (ab30731)参考文献

This product has been referenced in:
  • Munagala R  et al. Withaferin A induces p53-dependent apoptosis by repression of HPV oncogenes and upregulation of tumor suppressor proteins in human cervical cancer cells. Carcinogenesis : (2011). Read more (PubMed: 21859835) »
  • Clawson GA  et al. Intracellular localization of the tumor suppressor HtrA1/Prss11 and its association with HPV16 E6 and E7 proteins. J Cell Biochem 105:81-8 (2008). Read more (PubMed: 18452160) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products.

These two monoclonal antibodies recognize the same protein (Human Papillomavirus 16 (E7)) but are from different clones.

http://www.abcam.com/ab20191:

http://ww...

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We are not aware of publications having used ab30731.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"