概述

  • 产品名称
    人PAI1 ELISA试剂盒(SERPINE1)
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 9.6%
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Tissue, Cerebral Spinal Fluid
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.02 ng/ml
  • 范围
    0.078 ng/ml - 5 ng/ml
  • 回收率

    98 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.

    A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.

    This assay recognizes both natural and recombinant Human PAI-1.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human PAI1 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    PAI1 Microplate (12 x 8 well strips) 1 unit
    PAI1 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • 组织特异性
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 疾病相关
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 序列相似性
    Belongs to the serpin family.
  • 翻译后修饰
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1_HUMAN
    • Plasminogen activator inhibitor 1
    • Serpin E1
    • SERPINE1
    see all
  • 数据库链接

    应用

    Our Abpromise guarantee covers the use of ab108891 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent dilution.

    图片

    • Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit
      Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit

      Standard curve mean of duplicates (+/- SD) with background readings subtracted

    • Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit
      Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit

      PAI1 measured in various samples showing quantity (ng) per mL of tested sample

    • Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit
      Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit

      PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested

    实验方案

    文献

    This product has been referenced in:
    • Giacoia EG  et al. PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation. Mol Cancer Res 12:322-34 (2014). Read more (PubMed: 24464915) »
    • Jin R  et al. Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD. Nutrients 6:3187-201 (2014). Human . Read more (PubMed: 25111123) »

    See all 2 Publications for this product

    发表研究结果有使用 ab108891？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    6 reviews or Q&A

    10
    Votes: Highest First

    Product code: 100662
    Lot number: GR65556-1
    Inquiry: I want to do an VEGF Elisa (ab 100662), but can not find in what anti-coagulant I have to collect the plasma in? I also ordered an Elisa kit for PAI1 (ab108891, lot# GR69250-2)and there plasma...

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    Thank you for contacting us.

    I can confirm that Heparin should not be interfering with the assay using these kits.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

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    can kit be used with mouse samples?
    cross-reactivity of antibodies likely?

    Thank you for contacting us.

    I checked with the lab and they let me know that the homology with mouse is only 2%, thus they do not recommend using it for this species. It will most likely not work.

    Let me know if I can assist you f...

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    I am interested in measurements of total human and rabbit PAI-1 antigen in biological fluids. I have a question about your product ab108891 - how specific this kit is for human PAI-1? In other words, if I have a mixture of human and rabbit PAI-1, wheth...

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    Thank you for contacting Abcam regarding ab108891.


    Our human kit is specific for human PAI-1 total antigen measurement. It does not cross react with rabbit PAI-1. Unfortunately, we do not have a rabbit specific kit available.

    Read More

    I have a question about Plasminogen Activator Inhibitor 1 Human ELISA Kit (ab108891). PAI-1 is found in several forms:1.active, 2.inactive(latent), 3.complexed to tPA, 4.complexed to uPA. Which form of PAI-1 are measured in the kit ?

    Thank you for your enquiry.

    This kit measures the total PAi-1 including free PAI-1, PAI-tPA complexes and PAI-1-uPA complexes.

    I hope this information will be helpful.

    Hi Abcam,

    Could you please advise if these kits can be used to test citrate plasma?

    Thanks for your help!

    Kind regards,

    Thank you for contacting us.

    All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

    I hope this information has been of help. If your require any further information please do not...

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    The standards for ab108891 and ab108914 di not state how much standard was in the vial. The standards were supplied as lyophillsed powder but on ab108914 the standard vial said 2ng/ml and for ab108891, the vial stated 5ng/ml. Is there 2ng and 5ng in th...

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    Thank you for contacting Abcam today.

    I am sorry about the confusion over the labeling of the standards for ab108891 and ab108914. I have heard back from the lab about the correct concentrations for these standards:

    ab108891 - Recon...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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