概述

描述

  • 性质Synthetic

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技术指标

Our Abpromise guarantee covers the use of ab18623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 形式Liquid
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.

    Information available upon request.

常规信息

  • 别名
    • CAP20
    • CDK-interacting protein 1
    • CDKI
    • CDKN1
    • Cdkn1a
    • CDN1A_HUMAN
    • CIP1
    • Cyclin Dependent Kinase Inhibitor 1A
    • Cyclin-dependent kinase inhibitor 1
    • Cyclin-dependent kinase inhibitor 1A (P21)
    • Cyclin-dependent kinase inhibitor 1A (p21, Cip1)
    • DNA Synthesis Inhibitor
    • MDA-6
    • MDA6
    • Melanoma differentiation-associated protein
    • Melanoma differentiation-associated protein 6
    • p21
    • P21 protein
    • p21CIP1
    • p21Cip1/Waf1
    • p21WAF
    • PIC1
    • SDI1
    • SLC12A9
    • WAF1
    • Wild type p53 activated fragment 1
    • Wild type p53 activated fragment 1 (WAF1)
    • Wildtype p53-activated fragment 1
    see all
  • 功能May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
  • 组织特异性Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.
  • 序列相似性Belongs to the CDI family.
  • 结构域The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
  • 翻译后修饰Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt

Human p21 peptide (ab18623)参考文献

ab18623 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"