概述

  • 产品名称
    人Oncostatin M ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 126.2 117% - 136%
    Plasma 76.63 83% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Oncostatin M Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Oncostatin M in plasma and cell culture supernatants. (GeneCards: Osm) 


    Note: Human Oncostatin M concentration is pretty low in normal plasma, it may not be detected in this assay.


    We have not been able to detect endogenous Human Oncostatin M in normal serum with ab100619, only in serum spiked with Human Oncostatin M.


    This assay employs an antibody specific for Human Oncostatin M coated on a 96- well plate. Standards and samples are pipetted into the wells and Oncostatin M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Oncostatin M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Oncostatin M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


     

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 功能
    Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
  • 序列相似性
    Belongs to the LIF/OSM family.
  • 翻译后修饰
    Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • MGC20461
    • ONCM_HUMAN
    • Oncostatin M precursor
    • Oncostatin-M
    • OncostatinM
    • OSM
    • OTTMUSP00000005249
    • RP23-453B22.1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Oncostatin M ELISA Kit 图像

  • Representative Standard Curve using ab100619.

实验方案

Human Oncostatin M ELISA Kit (ab100619)参考文献

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). Read more (PubMed: 26732624) »
  • Croci DO  et al. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma. J Exp Med 209:1985-2000 (2012). Human . Read more (PubMed: 23027923) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to confirm that ab100619 Oncostatin M Human ELISA Kit has only been validated for serum, pasma, cell culture spernatants, and has not been tested with CSF.  However, this ELISA kit should be accommodating to a ...

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It is very difficult for us to recommend dilutions for kits like this since samples can have a huge range in the concentration of protein present depending on the samples. The best thing to do to is to run a representative samples in a serial dilution ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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