概述

  • 产品名称
    人Oncostatin M ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 回收率

    97 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 126.2 117% - 136%
    Plasma 76.63 83% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Oncostatin M Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Oncostatin M in plasma and cell culture supernatants. (GeneCards: Osm) 


    Note: Human Oncostatin M concentration is pretty low in normal plasma, it may not be detected in this assay.


    We have not been able to detect endogenous Human Oncostatin M in normal serum with ab100619, only in serum spiked with Human Oncostatin M.


    This assay employs an antibody specific for Human Oncostatin M coated on a 96- well plate. Standards and samples are pipetted into the wells and Oncostatin M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Oncostatin M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Oncostatin M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


     

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100619.

实验方案

文献

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). Read more (PubMed: 26732624) »
  • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). Human . Read more (PubMed: 27141367) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for your enquiry. I am sorry to confirm that ab100619 Oncostatin M Human ELISA Kit has only been validated for serum, pasma, cell culture spernatants, and has not been tested with CSF.  However, this ELISA kit should be accommodating to a ...

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It is very difficult for us to recommend dilutions for kits like this since samples can have a huge range in the concentration of protein present depending on the samples. The best thing to do to is to run a representative samples in a serial dilution ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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