概述

  • 产品名称

    人NRG1 beta 1 ELISA试剂盒

  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 50 pg/ml
  • 范围
    82.3 pg/ml - 20000 pg/ml
  • 复检

    99 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 114.9 102% - 123%
    Plasma 86.02 79% - 94%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human NRG1 beta 1 (lyophilized) 2 vials
    NRG1 beta 1 Microplate (12 x 8 wells) 1 unit
    Recombinant Human NRG1 beta 1 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
  • 组织特异性
    Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific.
  • 疾病相关
    Note=A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with ODZ4. The translocation fuses the 5'-end of ODZ4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines.
  • 序列相似性
    Belongs to the neuregulin family.
    Contains 1 EGF-like domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 发展阶段
    Detectable at early embryonic ages. Isoform 10 is highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons. Type IV isoforms are expressed in fetal brain.
  • 结构域
    The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • 翻译后修饰
    Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
  • 细胞定位
    Secreted; Cell membrane. Does not seem to be active; Membrane. May possess an internal uncleaved signal sequence; Nucleus. May be nuclear and Secreted. Has a signal peptide.
  • Information by UniProt
  • 别名
    • Acetylcholine receptor inducing activity
    • Acetylcholine receptor-inducing activity
    • ARIA
    • Breast cancer cell differentiation factor p45
    • GGF
    • GGF2
    • Glial growth factor
    • Heregulin
    • HGL
    • HRG
    • HRG1
    • HRGA
    • MST131
    • NDF
    • Neu differentiation factor
    • Neuregulin 1
    • Neuregulin-1
    • Neuregulin1
    • nrg1
    • NRG1 beta 1
    • NRG1_HUMAN
    • Pro neuregulin 1
    • Pro NRG1
    • Pro-NRG1
    • Sensory and motor neuron derived factor
    • Sensory and motor neuron-derived factor
    • SMDF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • NRG1 beta measured in culture supernatants, THP-1 levels below level of detection (duplicates +/- SD).

  • NRG1 beta measured in human biological fluids; signals from serum and from rat and mouse samples were below level of detection (duplicates +/- SD).

实验方案

文献

This product has been referenced in:
  • Cohen JE  et al. A bioengineered hydrogel system enables targeted and sustained intramyocardial delivery of neuregulin, activating the cardiomyocyte cell cycle and enhancing ventricular function in a murine model of ischemic cardiomyopathy. Circ Heart Fail 7:619-26 (2014). Sandwich ELISA . Read more (PubMed: 24902740) »

See 1 Publication for this product

客户评价及客户问答

1. It is unlikely that this kit will cross react with the EGF domain.
2. No cross reactivity information is available for mouse.

Thank you for your inquiry.

If using serum or plasma samples, use Assay Diluent A (Item D) to dilute 2-fold. Please note that this should only be used as a starting point and the optimal sample dilution will need to be determined by the end-...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

Unfortunately the diluted standards cannot be stored for use later.The only standard that can be st...

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Thank you for your inquiry.

The recommended serum/plasma dilution is 2-fold. Please note that this should only be used as a starting point and the optimal sample dilution will need to be determined by the end-user empirically.

I h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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