Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
neuronal Nitric Oxide Synthase
Nitric oxide synthase , neuronal, included
Nitric oxide synthase 1
Nitric oxide synthase 1 (neuronal)
Nitric oxide synthase, brain
Nitric oxide synthase, penile neuronal, included
NOS type I
Peptidyl-cysteine S-nitrosylase NOS1
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. Contains 1 PDZ (DHR) domain.
The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - nNOS (neuronal) peptide (1409-1424) - phospho S1416 (ab41773)This image is courtesy of an Abreview submitted by Jose Donato
Ab41773 (at concentrations of 0.25, 0.5 and 1 µg/ml) blocking the labeling of phsopho-nNOS S1416 by ab5583 (1/500) in mouse tissue sections (paraventricular nuclei of the hypotalamus) by immunohistochemistry (frozen sections). A Biotin-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.