人nNOS (neuronal) (phospho S1417)肽(ab41773)

概述

描述

  • 性质Synthetic
  • 氨基酸序列
    • AccessionP29475
    • 种属Human
    • 序列CNRLRSE(pS)IAFIEESK
    • 氨基酸1411 to 1425
    • 修饰phospho S1417

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技术指标

Our Abpromise guarantee covers the use of ab41773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Immunohistochemistry (Frozen sections)

    Neutralising

    competitive binding assays

    Blocking - Blocking peptide for Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

常规信息

  • 别名
    • 2310005C01Rik
    • BNOS
    • Constitutive NOS
    • EC 1.14.13.39
    • IHPS 1
    • IHPS1
    • N-NOS
    • NC-NOS
    • neuronal Nitric Oxide Synthase
    • Neuronal NOS
    • Nitric oxide synthase , neuronal, included
    • Nitric oxide synthase 1
    • Nitric oxide synthase 1 (neuronal)
    • Nitric oxide synthase, brain
    • Nitric oxide synthase, penile neuronal, included
    • NNOS
    • NO
    • NOS
    • NOS 1
    • NOS type I
    • NOS-I
    • NOS1
    • NOS1_HUMAN
    • Peptidyl-cysteine S-nitrosylase NOS1
    see all
  • 功能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 组织特异性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 序列相似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻译后修饰Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 细胞定位Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt

Human nNOS (neuronal) (phospho S1417) peptide 图像

  • Ab41773 (at concentrations of 0.25, 0.5 and 1 µg/ml) blocking the labeling of phsopho-nNOS S1416 by ab5583 (1/500) in mouse tissue sections (paraventricular nuclei of the hypotalamus) by immunohistochemistry (frozen sections). A Biotin-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

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Human nNOS (neuronal) (phospho S1417) peptide (ab41773)参考文献

This product has been referenced in:
  • Donato J  et al. Leptin Induces Phosphorylation of Neuronal Nitric Oxide Synthase in Defined Hypothalamic Neurons. Endocrinology : (2010). Read more (PubMed: 20881244) »

See 1 Publication for this product

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Application Other

Review text: Peptide used to block the phospho-nNOS S1416 labeling in mouse brain sections, using immunohistochemistry (peroxidase reaction using DAB).
It completely neutralized the phospho-nNOS labeling in the three concentrations used (1.0, 0.5 and 0.25 µg/mL). The dilution of the primary antibody was 1:500.
The results using this blocking peptide can be found in:
Donato J Jr, Frazão R, Fukuda M, Vianna C, Elias CF. Leptin induces phosphorylation of neuronal nitric oxide synthase in defined hypothalamic neurons. Endocrinology. Endocrinology, November 2010, 151(11):0000–0000.
Sample: Mouse Tissue sections

Primary antibody (in addition to 'nNOS (neuronal) peptide (1409-1424) - phospho S1416')
Primary antibody: Abcam primary antibody: Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)
Dilution: 1/500

Secondary antibody
Name: Non-Abcam antibody was used: donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution: 1/1000

Additional data
PubMed ID: 20881244: View
Username

Dr. Jose Donato

Verified customer

提交于 Oct 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"