人Neurotrophin 4 ELISA试剂盒(ab100616)

概述

  • 产品名称人Neurotrophin 4 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    1.4 pg/ml - 1000 pg/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101.5 87% - 110%
    Serum 98.98 85% - 108%
    Plasma 95.49 83% - 107%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Neurotrophin 4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 4 in serum, plasma, and cell culture supernatants. (Human Neurotrophin 4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human Neurotrophin 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Target-derived survival factor for peripheral sensory sympathetic neurons.
  • 组织特异性Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.
  • 疾病相关Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O) [MIM:613100]. A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
  • 序列相似性Belongs to the NGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • GLC10
    • GLC1O
    • Neurotrophic factor 4
    • Neurotrophic factor 5
    • Neurotrophin 5
    • Neurotrophin 5 (neurotrophin 4/5)
    • Neurotrophin-4
    • Neurotrophin-5
    • Neutrophic factor 4
    • Neutrophic factor 5
    • NT 4
    • NT 4/5
    • NT 5
    • NT-4
    • NT-5
    • NT4/5
    • NT4P
    • NT5
    • NTF4
    • NTF4_HUMAN
    • NTF5
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Neurotrophin 4 ELISA Kit 图像

  • Representative Standard Curve using ab100616

  • Representative Standard Curve using ab100616

实验方案

Human Neurotrophin 4 ELISA Kit (ab100616)参考文献

ab100616 has not yet been referenced specifically in any publications.

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