The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-NCE2 antibody (ab15707)
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
NEDD8 carrier protein UBE2F
NEDD8 conjugating enzyme
NEDD8 conjugating enzyme UBE2F
NEDD8 protein ligase UBE2F
NEDD8-conjugating enzyme 2
NEDD8-conjugating enzyme UBE2F
Ubiquitin conjugating enzyme E2F
Ubiquitin conjugating enzyme E2F (putative)
Ubiquitin-conjugating enzyme E2 F
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Widely expressed (at protein level).
Protein modification; protein neddylation.
Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.