概述

  • 产品名称人NBL1 ELISA试剂盒
    参阅全部 NBL1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 35 pg/ml
  • 范围
    27.43 pg/ml - 20000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 111.3 107% - 122%
    Serum 100.1 91% - 109%
    Plasma 81.82 72% - 90%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s NBL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm..

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
  • 组织特异性Most abundant in normal lung and meningioma.
  • 序列相似性Belongs to the DAN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • DAN
    • DAN domain family member 1
    • DAND 1
    • DAND1
    • Differential screening selected gene aberrant in neuroblastoma
    • MGC8972
    • NB
    • NBL 1
    • NBL1
    • NBL1_HUMAN
    • Neuroblastoma candidate region suppression of tumorigenicity 1
    • Neuroblastoma suppression of tumorigenicity 1
    • Neuroblastoma suppressor of tumorigenicity 1
    • NO 3
    • NO3
    • Protein N03
    • Zinc finger protein DAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human NBL1 ELISA Kit 图像

  • Representative Standard Curve using ab99997

  • Representative Standard Curve using ab99997

实验方案

Human NBL1 ELISA Kit (ab99997)参考文献

ab99997 has not yet been referenced specifically in any publications.

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