人Myeloperoxidase ELISA试剂盒(MPO) (ab119605)

概述

  • 产品名称人Myeloperoxidase ELISA试剂盒(MPO)
    参阅全部 Myeloperoxidase 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Cell Lysate, Tissue Homogenate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    312 pg/ml - 20000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Myeloperoxidase (MPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myeloperoxidase in cell culture supernatants, cell lysate, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine.

    A Myeloperoxidase specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Myeloperoxidase is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Myeloperoxidase amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • 疾病相关Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • 序列相似性Belongs to the peroxidase family. XPO subfamily.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 别名
    • 84 kDa myeloperoxidase
    • 89 kDa myeloperoxidase
    • EC 1.11.1.7
    • EC1.11.2.2
    • fj80f04
    • MPO
    • mpx
    • myeloid-specific peroxidase
    • Myeloperoxidase
    • Myeloperoxidase heavy chain
    • Myeloperoxidase light chain
    • PERM_HUMAN
    • wu:fj80f04
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Myeloperoxidase ELISA Kit (MPO) 图像

  • Representative Standard Curve using ab119605.

实验方案

Human Myeloperoxidase ELISA Kit (MPO) (ab119605)参考文献

This product has been referenced in:
  • Chu Y  et al. Myeloperoxidase is increased in human cerebral aneurysms and increases formation and rupture of cerebral aneurysms in mice. Stroke 46:1651-6 (2015). Read more (PubMed: 25922506) »
  • Zhou C  et al. Decreased progranulin levels in patients and rats with subarachnoid hemorrhage: a potential role in inhibiting inflammation by suppressing neutrophil recruitment. J Neuroinflammation 12:200 (2015). Rat . Read more (PubMed: 26527034) »

See all 2 Publications for this product

Product Wall

Abreviews
Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
The concentrations of MPO in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab119605) according to the manufacturer´s protocol. As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: 1:2, 1:10, 1:50, 1:300, 1:1500.
The same amount of total protein from 8 uterine aspirates was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 4-13% (average of 7%). Concentration of MMP9 of uterine aspirates ranged from 2 to 1053 ng/ul (see figure 1B).
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Verified customer

提交于 Apr 18 2016

Thank you for contacting us. Cross-reactivity with other molecules such as VPO has not been tested, however the homology between the immunogen of the antibody in the kit and human VPO is 41%, much to low for cross-reactivity to occurs. Typically around...

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Thank you for your enquiry.

I can confirm that this kit (ab119605: MPO Human ELISA Kit) has been tested for cross-reactivity with human samples. It doesn't work on mouse samples.

If you need any further assistance in the future, ple...

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