人MMP7 ELISA试剂盒(ab100608)

概述

  • 产品名称人MMP7 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.15 ng/ml
  • 范围
    0.137 ng/ml - 100 ng/ml
  • 复检

    90 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 90.52 82% - 102%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MMP7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP7 in cell culture supernates.

    This assay employs an antibody specific for Human MMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • 序列相似性Belongs to the peptidase M10A family.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • Matrilysin
    • Matrin
    • Matrix metalloproteinase 7
    • Matrix metalloproteinase-7
    • MMP-7
    • MMP7
    • MMP7_HUMAN
    • MPSL1
    • Pump 1 protease
    • Pump-1 protease
    • PUMP1
    • Uterine metalloproteinase
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MMP7 ELISA Kit 图像

  • Representative Standard Curve using ab100608.

实验方案

Human MMP7 ELISA Kit (ab100608)参考文献

ab100608 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that kits ab100606 and ab100608 recognizes both pro and mature forms of respective proteins.

I now have further information to share from the lab in regards to the MMP7 Human ELISA Kit (ab100608).

The lab have further confirmed that the results obtained with the lot numbers GR88563 & GR98815 have performed as expected: standard cu...

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I am sorry for the delay in getting back to you. I now have some further input form the lab regarding the MMP7 Human ELISA Kit (ab100608) your customer has been having some difficulty with.

The first lot tested does contain good linearity and...

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I am sorry to hear your customer has been experiencing difficulties with the MMP7 Human ELISA Kit (ab100608). We take product complaints very seriously, and investigate every product that we feel may not be perfo...

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