人MMP3 ELISA试剂盒(ab100607)

概述

  • 产品名称人MMP3 ELISA试剂盒
    参阅全部 MMP3 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.3 ng/ml
  • 范围
    0.41 ng/ml - 100 ng/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.72 84% - 103%
    Serum 95.39 83% - 103%
    Plasma 96.25 85% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP3 pro and active forms in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).

    This assay employs an antibody specific for Human MMP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • CHDS6
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Proteoglycanase
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelysin 1
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MMP3 ELISA Kit 图像

  • Representative Standard Curve using ab100607.

实验方案

Human MMP3 ELISA Kit (ab100607)参考文献

ab100607 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for clarifying this so quickly.
I canceled the order with the free of charge replacement and edited a credit note reference CN ********.
Sorry for my mistake and thank you for being so quick with your reply.

Thank you for kindly confirming these details.
As requested, we arranged a free of charge replacement (2 units of kit ab100607).
Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

Thank you for your email.

I am happy to process the replacement. Could you please confirm how many kits (ab100607) your customer ordered originally?

Thank you.

Thank you very much for confirming all these details. This is indeed very useful for us.
It looks like the customer has received a kit with a faulty HRP / HRP conjugate. We will therefore be happy to replace the kit for the customer. Please let us...

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rmmarize the problem. I am sorry to hear that this kit is not providing satisfactory results.
We have not yet received any negative feedback and I am not able yet to tell where the problem might is coming from. I would therefore appreciate if you ...

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Thank you for your interest in the MMP-3 and IL23 kits.

The volume of sample required for each test is 100ul per well of the assay plate.

Regarding the 100% discount, I want to be sure you understand the offer, which is also descr...

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Abreview for MMP3 Human ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Human Cell (Skin fibroblasts)
Specification: Skin fibroblasts
Amount of Sample: 200 µg
Type of Kit Used: ELISA
Positive control: Included in kit as part of standard curve.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Incubation times are a little longer than in some other kits, which must be remembered when planning multiple experiments, but the data generated is of a better quality overall.

The graph shows the differing levels of MMP3 released by primary fibroblasts exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.

(Sorry I cant say what the actives actually are due to business nature of the work).
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Abcam user community

Verified customer

提交于 Feb 09 2012

Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"