• 产品名称人MMP2 ELISA试剂盒
    参阅全部 MMP2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3.5 ng/ml
  • 范围
    3.3 ng/ml - 800 ng/ml
  • 复检

    81 %

    样品类型 平均% 范围
    Cell culture supernatant 77.25 69% - 92%
    Serum 81.27 70% - 93%
    Plasma 84.53 72% - 95%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human MMP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP2 in serum, plasma, and cell culture supernates. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties).

    This assay employs an antibody specific for Human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate


  • 功能Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • 组织特异性Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • 疾病相关Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • 细胞定位Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • 别名
    • 72 kDa gelatinase
    • 72 kDa type IV collagenase
    • CLG 4
    • CLG 4A
    • CLG4
    • CLG4A
    • Collagenase type IV A
    • EC
    • Gelatinase A
    • Gelatinase alpha
    • Gelatinase neutrophil
    • Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • Matrix metalloproteinase 2
    • Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)
    • Matrix metalloproteinase II
    • Matrix metalloproteinase-2
    • MMP 2
    • MMP II
    • MMP-2
    • MMP2
    • MMP2_HUMAN
    • MONA
    • Neutrophil gelatinase
    • PEX
    • TBE 1
    • TBE-1
    see all
  • 数据库链接


Our Abpromise guarantee covers the use of ab100606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MMP2 ELISA Kit 图像

  • MMP2 measured in biological fluids showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab100606.


Human MMP2 ELISA Kit (ab100606)参考文献

This product has been referenced in:
  • Cao L  et al. MiR-324-5p Suppresses Hepatocellular Carcinoma Cell Invasion by Counteracting ECM Degradation through Post-Transcriptionally Downregulating ETS1 and SP1. PLoS One 10:e0133074 (2015). Read more (PubMed: 26177288) »
  • Zhao W  et al. In Vitro Antimetastatic Effect of Phosphatidylinositol 3-Kinase Inhibitor ZSTK474 on Prostate Cancer PC3 Cells. Int J Mol Sci 14:13577-91 (2013). Read more (PubMed: 23812078) »

See all 2 Publications for this product

Product Wall

I can confirm that kits ab100606 and ab100608 recognizes both pro and mature forms of respective proteins.

MMP2 Human ELISA kit works great

Excellent Excellent 5/5 (Ease of Use)
This kit was very easy to use. I followed the product insert exactly and it worked very well. The results were also reproducible. I would use abcam again for my ELISA or western blotting needs.

Abcam user community

Verified customer

提交于 Jun 18 2013

Thank you for your inquiry.

I can confirm that these two kits have not been experimentally tested with human cerebrospinal fluid and are therefore not guaranteed to work with this kind of sample.

But we are confident that ab100606 a...

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Thank you for contacting us.

I now have further information to share with you in regards to the MMP2 ELISA kits, ab100606 and ab100730. The antibodies used within these kits have used the following immunogens:

ab100606 capture antib...

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Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

Thank you very much for your kind reply.

I am currently having discussions with developer of this kit regarding the new kit we agreed, sending as a free of charge replacement. This is an extra step for avoiding any further inconvenience due t...

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This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made...

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Thank you for contacting us. I apologize for the delay.

We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping c...

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Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very ...

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Thank you very much for contacting. I apologize for the delay as we were conducting some tests.

We have also seen gloopy things in the Assay buffer which first seen as like fungal growth however when we warmed the buffer at room temperature w...

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