为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
|Cell culture supernatant||94.72||84% - 103%|
|Serum||91.99||82% - 102%|
|Plasma||93.13||83% - 103%|
Abcam’s MMP13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MMP13 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The kit detects total MMP-13 levels.
Optimisation may be required with urine samples.
|组件||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|700X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human MMP13||2 vials|
|MMP13 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MMP13 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
MMP13 measured in cell supernatanses showing quantity (pg) per mL of tested sample
Representative standard curve using ab100605