概述

  • 产品名称人MMP13 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.72 84% - 103%
    Serum 91.99 82% - 102%
    Plasma 93.13 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MMP13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MMP13 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    The kit detects total MMP-13 levels.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 组织特异性Seems to be specific to breast carcinomas.
  • 疾病相关Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • CLG 3
    • CLG3
    • Collagenase 3
    • Collagenase3
    • MANDP1
    • Matrix metallopeptidase 13 (collagenase 3)
    • Matrix Metalloproteinase 13
    • Matrix metalloproteinase-13
    • MMP 13
    • MMP-13
    • Mmp13
    • MMP13_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MMP13 ELISA Kit 图像

  • MMP13 measured in cell supernatanses showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100605

实验方案

Human MMP13 ELISA Kit (ab100605)参考文献

This product has been referenced in:
  • Collins JA  et al. Oxygen and pH-sensitivity of human osteoarthritic chondrocytes in 3-D alginate bead culture system. Osteoarthritis Cartilage 21:1790-8 (2013). ELISA ; Human . Read more (PubMed: 23850530) »
  • Jayasuriya CT  et al. Matrilin-3 Induction of IL-1 receptor antagonist Is required for up-regulating collagen II and aggrecan and down-regulating ADAMTS-5 gene expression. Arthritis Res Ther 14:R197 (2012). ELISA . Read more (PubMed: 22967398) »

See all 2 Publications for this product

Product Wall

Abreviews
I tested this kit for cross-reactivity with bovine and porcine MMP-13 using bovine standards and a porcine positive control sample from a USBio bovine MMP-13 ELISA kit. The kit did not detect bovine or porcine samples (very low or negative OD values).
The human standards seemed to work well, and the standard diluent also seems to be important because human standards in the bovine diluent produced much lower signals.
Username

Ms. Ann Ouyang

Verified customer

提交于 Apr 08 2015

For plasma:
Collect blood into EDTA or Sodium heparin tube (e.g. BD vacutainer, Cat. No: 8001302 or 16852)

Spin 10 minutes at 3000 rpm

Aliquot into small tubes and store at –800C until use.

For serum:

Colle...

Read More

Thank you for contacting Abcam. I have contacted the lab and they have confirmed that the MMP13 Human ELISA kit (ab100605) recognizes total MMP-13 levels. If there is anything else that I can do, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"