人MMP10 ELISA试剂盒(ab100602)

概述

  • 产品名称人MMP10 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    2.06 pg/ml - 1500 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.31 87% - 109%
    Serum 96.54 85% - 108%
    Plasma 94.57 84% - 105%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MMP10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP10 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernates.

    This assay employs an antibody specific for Human MMP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • AV377895
    • Matrix Metalloproteinase 10
    • Matrix metalloproteinase-10
    • MMP 10
    • MMP-10
    • Mmp10
    • MMP10_HUMAN
    • SL 2
    • SL-2
    • SL2
    • STMY2
    • Stromelysin 2
    • Stromelysin II
    • Stromelysin-2
    • Stromelysin2
    • Transin 2
    • Transin-2
    • Transin2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MMP10 ELISA Kit 图像

  • Representative Standard Curve using ab100602.

实验方案

Human MMP10 ELISA Kit (ab100602)参考文献

ab100602 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab100602. I can confirm that this kit will recognize both pro and active forms of MMP10 protein. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"