概述

  • 产品名称
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101.5 91% - 112%
    Serum 105.3 92% - 115%
    Plasma 98.23 90% - 114%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MIP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP5 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MIP5 2 vials
    MIP5 Microplate (12 strips x 8 wells) 1 unit
    Recombinant Human MIP5 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
  • 组织特异性
    Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
  • 序列相似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • C-C motif chemokine 15
    • C-C motif chemokine ligand 15
    • CC chemokine 3
    • CCL15
    • CCL15(29-92)
    • CCL15_HUMAN
    • Chemokine (C C motif) ligand 15
    • Chemokine CC-2
    • Chemokine CC2
    • HCC-2
    • HCC2
    • HMRP 2B
    • HMRP2B
    • Leukotactin 1
    • Leukotactin-1
    • LKN-1
    • LKN1
    • Macrophage inflammatory protein 5
    • MIP 1D
    • MIP-1 delta
    • MIP-1D
    • MIP-5
    • MIP1 delta
    • MIP1d
    • MIP5
    • MRP 2B
    • Mrp-2b
    • Mrp2b
    • NCC 3
    • NCC-3
    • NCC3
    • New CC chemokine 3
    • OTTHUMP00000163955
    • SCYA15
    • SCYL3
    • Small inducible cytokine A15
    • Small inducible cytokine subfamily A (Cys Cys), member 15
    • Small-inducible cytokine A15
    • SY15
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100598.

  • Representative Standard Curve using ab100598.

实验方案

文献

ab100598 has not yet been referenced specifically in any publications.

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