人MIP3a ELISA试剂盒(CCL20) (ab100600)

概述

  • 产品名称人MIP3a ELISA试剂盒(CCL20)
    参阅全部 Macrophage Inflammatory Protein 3 alpha 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture extracts, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    0.8 pg/ml - 600 pg/ml
  • 复检

    91 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture extracts 89.75 78% - 102%
    Tissue Extracts 93.28 81% - 105%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MIP3a (CCL20) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3a in cell lysates and tissue lysates.

    This assay employs an antibody specific for Human MIP3a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • 组织特异性Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Beta-chemokine exodus-1
    • C C motif chemokine ligand 20
    • C-C motif chemokine 20
    • CC chemokine LARC
    • Ccl20
    • CCL20(2-70)
    • CCL20_HUMAN
    • Chemokine (C C motif) ligand 20
    • Chemokine CC motif ligand 20
    • CKb4
    • Exodus
    • Exodus 1
    • LARC
    • Liver and activation-regulated chemokine
    • Macrophage inflammatory protein 3 alpha
    • MIP 3 alpha
    • MIP 3A
    • MIP-3-alpha
    • MIP-3a
    • MIP3A
    • SCYA20
    • Small inducible cytokine A20
    • Small inducible cytokine subfamily A (Cys Cys) member 20
    • Small-inducible cytokine A20
    • ST38
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MIP3a ELISA Kit (CCL20) 图像

  • Representative Standard Curve using ab100600.

实验方案

Human MIP3a ELISA Kit (CCL20) (ab100600)参考文献

ab100600 has not yet been referenced specifically in any publications.

Product Wall

Increasing the loading volume per well could increase the signal so this certainly could have been a contributing factor in the high background response.

In future experiments, in addition to ensuring the correct loading volume, the customer s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"