人MIP3a ELISA试剂盒(CCL20) (ab100599)

概述

  • 产品名称
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    0.8 pg/ml - 600 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.25 83% - 102%
    Serum 95.39 83% - 103%
    Plasma 93.88 82% - 102%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MIP3a Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3a in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human MIP3a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human MIP3a ELISA Kit (CCL20) (ab178015) from our SimpleStep ELISA® range.


     

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MIP3a 2 vials
    MIP3a Microplate (12 strips x 8 wells) 1 unit
    Recombinant Human MIP3a Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213.
  • 组织特异性
    Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon.
  • 序列相似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰
    C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Beta-chemokine exodus-1
    • C C motif chemokine ligand 20
    • C-C motif chemokine 20
    • CC chemokine LARC
    • Ccl20
    • CCL20(2-70)
    • CCL20_HUMAN
    • Chemokine (C C motif) ligand 20
    • Chemokine CC motif ligand 20
    • CKb4
    • Exodus
    • Exodus 1
    • LARC
    • Liver and activation-regulated chemokine
    • Macrophage inflammatory protein 3 alpha
    • MIP 3 alpha
    • MIP 3A
    • MIP-3-alpha
    • MIP-3a
    • MIP3A
    • SCYA20
    • Small inducible cytokine A20
    • Small inducible cytokine subfamily A (Cys Cys) member 20
    • Small-inducible cytokine A20
    • ST38
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab100599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100599.

  • Representative Standard Curve using ab100599.

实验方案

文献

ab100599 has not yet been referenced specifically in any publications.

客户评价及客户问答

CCL20-MIP3a secretion by human macrophages & DC

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL20-MIP3a human macrophage and DC secretion in culture for 3 and 7 days, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by CCL3 secretion by M1 macrophages subtyes, particularly at day 3.
M0 = MCSF M1 = MCSF+IFNg M2a = MCSF+IL4 M2c = MCSF+TGFb or MCSF+IL10 or MCSF+Dexamethasone DC = GMCSF+IL4.
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提交于 May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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