人MIP1a ELISA试剂盒(CCL3) (ab100596)

概述

  • 产品名称人MIP1a ELISA试剂盒(CCL3)
    参阅全部 Macrophage Inflammatory Protein 1 alpha / CCL3 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 6 pg/ml
  • 范围
    4.12 pg/ml - 3000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 125.2 120% - 132%
    Serum 87.12 74% - 93%
    Plasma 97.15 81% - 112%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MIP1a (CCL3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1a in serum, plasma (recommended using EDTA or Heparin plasma), and cell culture supernatants.

    This assay employs an antibody specific for Human MIP1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C C motif chemokine 3
    • CCL 3
    • CCL3
    • CCL3_HUMAN
    • Chemokine C C motif ligand 3
    • G0/G1 switch regulatory protein 19 1
    • G0/G1 switch regulatory protein 19-1
    • G0S19 1
    • G0S19 1 protein
    • Heparin binding chemotaxis protein
    • L2G25B
    • LD78 alpha
    • LD78-alpha(4-69)
    • LD78alpha
    • Macrophage inflammatory protein 1 alpha
    • Macrophage inflammatory protein 1-alpha
    • MIP 1 alpha
    • MIP 1A
    • MIP-1-alpha
    • MIP-1-alpha(4-69)
    • MIP1A
    • PAT 464.1
    • SCYA 3
    • SCYA3
    • SIS alpha
    • SIS beta
    • SIS-beta
    • Small inducible cytokine A3
    • small inducible cytokine A3 (homologous to mouse Mip-1a)
    • Small-inducible cytokine A3
    • Tonsillar lymphocyte LD78 alpha protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MIP1a ELISA Kit (CCL3) 图像

  • Representative Standard Curve using ab100596.

实验方案

Human MIP1a ELISA Kit (CCL3) (ab100596)参考文献

ab100596 has not yet been referenced specifically in any publications.

Product Wall

CCL3-MIP1a human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL3-MIP1a human macrophage secretion in culture for 3 and 7 days, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by CCL3 secretion by M1, M2a & M2c-DEX macrophages subtyes, particularly at day 7.
M0 = MCSF M1 = MCSF+IFNg M2a = MCSF+IL4 M2c = MCSF+TGFb or MCSF+IL10 or MCSF+Dexamethasone.
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提交于 May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"