人MIG ELISA试剂盒(CXCL9) ELISA试剂盒(ab100595)

概述

  • 产品名称人MIG ELISA试剂盒(CXCL9) ELISA试剂盒
    参阅全部 CXCL9 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 101.3 89% - 108%
    Serum 94.56 82% - 102%
    Plasma 96.32 84% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MIG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIG in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIG coated on a 96-well plate. Standards and samples are pipetted into the wells and MIG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C-X-C motif chemokine 9
    • Chemokine (C-X-C motif) ligand 9
    • Chemokine, CXC motif, ligand 9
    • CMK
    • Crg 10
    • CXCL9
    • CXCL9_HUMAN
    • Gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • Monokine induced by gamma interferon
    • Monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • Small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MIG ELISA Kit (CXCL9) ELISA Kit 图像

  • Representative Standard Curve using ab100595.

  • Representative Standard Curve using ab100595.

实验方案

Human MIG ELISA Kit (CXCL9) ELISA Kit (ab100595)参考文献

ab100595 has not yet been referenced specifically in any publications.

Product Wall

MIG-CXCL9 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
MIG-CXCL9 secretion by human macrophages isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr, good specificity as shown by higher secretion by M1 macrophages (MSCF+IFNg) compared to the others subtypes (M0=MCSF M2=MSCF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)
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Abcam user community

Verified customer

提交于 Apr 01 2014

Following up my e-mail from yesterday, the lab has requested some information that will help us assess the issue.

In addition to the raw data, could you please tell us:

At what temperature were the kits stored and for how long? Read More

Thank you for contacting us and bringing this to our attention. For our records, can you please tell me again the test you described that indicated the antibodies in these kits are likely not the issue? Have you tested any samples other than the standa...

Read More

Thank you for your call last week and for your patience while I have been intouch with the lab regarding your enquiry.

I've heard back from the lab, and there is no BSA or anything else in the wash buffer that we would expect to produce highe...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"