人MICB ELISA试剂盒(ab100593)

概述

  • 产品名称人MICB ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 75 pg/ml
  • 范围
    0.069 ng/ml - 50 ng/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.22 91% - 125%
    Serum 113.1 102% - 122%
    Plasma 75.47 65% - 88%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MICB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MICB coated on a 96-well plate. Standards and samples are pipetted into the wells and MICB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • 组织特异性Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue.
  • 疾病相关Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=The MICB*004 allele is associated with rheumatoid arthritis.
    Note=Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk.
  • 序列相似性Belongs to the MHC class I family. MIC subfamily.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 翻译后修饰Proteolytically cleaved and released from the cell surface of tumor cells.
  • 细胞定位Cell membrane. Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum.
  • Information by UniProt
  • 别名
    • MHC class I chain related protein B
    • MHC class I like molecule PERB11.2 IMX
    • MHC class I mic B antigen
    • MHC class I polypeptide related sequence B
    • MHC class I polypeptide-related sequence B
    • MIC B
    • MIC-B
    • MICB
    • MICB_HUMAN
    • PERB11.2
    • Stress inducible class I homolog
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MICB ELISA Kit 图像

  • Representative Standard Curve using ab100593.

  • Representative Standard Curve using ab100593.

实验方案

Human MICB ELISA Kit (ab100593)参考文献

ab100593 has not yet been referenced specifically in any publications.

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