概述

  • 产品名称人MICA ELISA试剂盒
    参阅全部 MICA 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 6 2214.6pg/ml 50.3 2.3%
    B 6 1102.1pg/ml 39.5 3.6%
    C 6 641.6pg/ml 47.7 7.4%
    批次间
    样品 n Mean SD CV%
    A 18 2416pg/ml 187.1 7.7%
    B 16 1200pg/ml 105.2 8.8%
    C 18 627pg/ml 50.9 8.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 123 pg/ml
  • 范围
    156 pg/ml - 5000 pg/ml
  • 检测时间
    3h 50m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MICA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of MHC class 1 chain-related gene A glycoprotein, in cell culture supernatants and buffered solutions

    A monoclonal antibody specific for MICA has been coated onto the wells of the microtiter strips provided. Samples, including standards of known MICA concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for MICA are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of MICA present in the samples.

    This kit will recognize both endogenous and recombinant Human MICA.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 标识符 1 x 96 tests 2 x 96 tests
    1X Standard Diluent Buffer 1 x 25ml 2 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-MICA (MHC Class I alpha) Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    MICA Microplate (12 x 8 well strips) 1 unit 2 units
    MICA Standard (lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • 研究领域
  • 相关性The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
  • 细胞定位Plasma membrane
  • 别名
    • FLJ60820
    • HLA class I antigen
    • MGC111087
    • MHC class I chain related gene A protein
    • MHC class I chain related protein A
    • MHC class I polypeptide related sequence A
    • MHC class I related protein
    • MIC A
    • PERB11.1
    • Stress inducible class I homolog
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab59569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MICA ELISA Kit 图像

实验方案

Human MICA ELISA Kit (ab59569)参考文献

ab59569 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patient. Both products are suitable to use with soluble MICA.  However, please notice there are slightly differences between the kits. Ab100592 has been tested in human fluids, as Urine, Serum, and Plasma and also in Cell culture sup...

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The clones mentionned below are not included in the Human MICA ELISA Kit.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"