概述

  • 产品名称人MICA ELISA试剂盒
    参阅全部 MICA 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    13.72 pg/ml - 10000 pg/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 116.9 109% - 124%
    Serum 112.3 95% - 129%
    Plasma 93.83 75% - 107%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MICA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MICA in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MICA coated on a 96-well plate. Standards and samples are pipetted into the wells and MICA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MICA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MICA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性The MHC class I chain-related (MIC) proteins are related to the Major histocompatibility complex (MHC) class I proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. The MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. MICA is present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
  • 细胞定位Plasma membrane
  • 别名
    • FLJ60820
    • HLA class I antigen
    • MGC111087
    • MHC class I chain related gene A protein
    • MHC class I chain related protein A
    • MHC class I polypeptide related sequence A
    • MHC class I related protein
    • MIC A
    • PERB11.1
    • Stress inducible class I homolog
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MICA ELISA Kit 图像

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • MICA measured in undiluted cell culture supernatants, U937 and THP-1 signals were below level of detection (13.7 pg x mL-1) (duplicates +/- SD).

  • MICA measured in undiluted human biological fluids; signals in rat and mouse samples were below level of detection (13.7 pg x mL-1) (duplicates +/- SD).

  • Representative Standard Curve using ab100592.

实验方案

Human MICA ELISA Kit (ab100592)参考文献

This product has been referenced in:
  • Ren J  et al. Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. Cell Mol Immunol N/A:N/A (2014). Read more (PubMed: 25363527) »

See 1 Publication for this product

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Please find below the answers from our collaborator to your questions:

1) Extracellular domain of MICA not detected. Is the standard the recombinant full length MICA protein or a fragment?
The antibody...

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Thank you for your patient. Both products are suitable to use with soluble MICA.  However, please notice there are slightly differences between the kits. Ab100592 has been tested in human fluids, as Urine, Serum, and Plasma and also in Cell culture sup...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"