概述

  • 产品名称人MER ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Cell Lysate, Tissue Homogenate
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    156 pg/ml - 10000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human MER in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MER in cell culture supernatants, cell lysates, tissue homogenates and serum.

    A MER specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for MER is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MER amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能In case of filovirus infection, seems to function as a cell entry factor.
  • 组织特异性Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
  • 疾病相关Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • C mer
    • c mer proto oncogene tyrosine kinase
    • MER receptor tyrosine kinase
    • Mertk
    • MERTK_HUMAN
    • Proto-oncogene c-Mer
    • Receptor tyrosine kinase MerTK
    • RP38
    • STK kinase
    • Tyrosine-protein kinase Mer
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MER ELISA Kit 图像

  • Representative standard curve using ab119604

实验方案

Human MER ELISA Kit (ab119604)参考文献

ab119604 has not yet been referenced specifically in any publications.

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