人MCP3 ELISA试剂盒(ab100588)

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概述

  • 产品名称
    人MCP3 ELISA试剂盒
    参阅全部 MCP3 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    24.58 pg/ml - 6000 pg/ml
  • 回收率

    95 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 88.99 79% - 98%
    Plasma 95.19 88% - 106%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MCP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MCP3 in plasma, and cell culture supernatants. (Human MCP3 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human MCP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human MCP3 in normal serum with ab100588, only in serum spiked with Human MCP3.


    Get higher sensitivity in only 90 minutes with Human MCP3 ELISA Kit (CCL7) (ab193769) from our SimpleStep ELISA® range.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

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应用

Our Abpromise guarantee covers the use of ab100588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve Using ab100588.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve Using ab100588.

实验方案

文献

ab100588 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab100588？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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Hello, I was wondering if  you happened to have a protocol for running the MCP-1 and MCP-3 ELISA assays on a DSX analyzer.  It looks as though it can be done, but I have concerns regarding the reagent dilutions, and would rather have a protocol that ha...

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Thank you for contacting us. Unfortunately the ELISA assays have not been used in conjunction with a DSX analyser (and we therefore do not have any specific protocols for this) but as long as the machine can accurately carry out the protocol as stated ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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