概述

  • 产品名称人MCP2 ELISA试剂盒
    参阅全部 MCP2 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    0.82 pg/ml - 200 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.25 85% - 105%
    Serum 93.88 84% - 104%
    Plasma 91.63 82% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MCP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP2 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MCP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MCP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • 组织特异性Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • C C motif chemokine 8
    • CCL 8
    • Ccl8
    • CCL8_HUMAN
    • Chemokine (C C motif) ligand 8
    • Chemokine ligand 8
    • HC14
    • MCP 2
    • MCP-2
    • MCP-2(6-76)
    • Monocyte chemoattractant protein 2
    • Monocyte chemotactic protein 2
    • SCYA10
    • SCYA8
    • Small inducible cytokine A8
    • Small inducible cytokine subfamily A (Cys Cys) member 8
    • Small-inducible cytokine A8
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human MCP2 ELISA Kit 图像

  • Representative Standard Curve using ab100587

  • Representative Standard Curve using ab100587

实验方案

Human MCP2 ELISA Kit (ab100587)参考文献

ab100587 has not yet been referenced specifically in any publications.

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