人Macrophage Stimulating Protein ELISA试剂盒(ab100612)

概述

  • 产品名称
    人Macrophage Stimulating Protein ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 8 pg/ml
  • 范围
    8.23 pg/ml - 2000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.25 86% - 106%
    Serum 94.48 83% - 104%
    Plasma 95.29 82% - 105%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Macrophage Stimulating Protein Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Macrophage Stimulating Protein in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Macrophage Stimulating Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Stimulating Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Macrophage Stimulating Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Stimulating Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 相关性
    Macrophage Stimulating Protein contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
  • 别名
    • D3F15S2
    • D3F15S2h
    • D9H3F15S2
    • DNF15S2
    • DNF15S2h
    • Hepatocyte growth factor like protein
    • Hepatocyte growth factor like protein homolog
    • HGFL
    • Macrophage stimulating 1
    • Macrophage stimulatory protein
    • MSP
    • MST1
    • NF15S2
    see all

应用

Our Abpromise guarantee covers the use of ab100612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Macrophage Stimulating Protein ELISA Kit 图像

  • Representative Standard Curve using ab100612.

  • Representative Standard Curve using ab100612.

实验方案

Human Macrophage Stimulating Protein ELISA Kit (ab100612)参考文献

ab100612 has not yet been referenced specifically in any publications.

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Thank you for contacting us.

Yes, EDTA, Heparin, or Citrate collected plasma can be used with ab100612.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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