概述

  • 产品名称人Mac-2BP ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.5%
    批次间
    样品 n Mean SD CV%
    Overall 11.9%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.92 ng/ml
  • 范围
    12.5 ng/ml - 200 ng/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 116 95% - 139%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Mac-2BP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Mac-2BP concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    Mac-2BP specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated Mac-2BP detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Mac-2BP amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • 组织特异性Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • 序列相似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • 细胞定位Secreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • 90K
    • Basement membrane autoantigen p105
    • BTBD17B
    • Galectin-3-binding protein
    • L3 antigen
    • Lectin galactoside-binding soluble 3-binding protein
    • LG3BP_HUMAN
    • LGALS3BP
    • MAC 2 BP
    • Mac-2 BP
    • Mac-2-binding protein
    • MAC2BP
    • Serum protein 90K
    • Tumor-associated antigen 90K
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Mac-2BP ELISA Kit 图像

  • Representative Standard Curve using ab119502.

实验方案

Human Mac-2BP ELISA Kit (ab119502)参考文献

ab119502 has not yet been referenced specifically in any publications.

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