概述

  • 产品名称
    人Mac-2BP ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.5%
    批次间
    样品 n Mean SD CV%
    Overall 11.9%
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.92 ng/ml
  • 范围
    12.5 ng/ml - 200 ng/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 116 95% - 139%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Mac-2BP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Mac-2BP concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    Mac-2BP specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated Mac-2BP detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Mac-2BP amount of sample captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119502.

实验方案

文献

ab119502 has not yet been referenced specifically in any publications.

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