人M-CSF ELISA试剂盒(ab100590)

概述

  • 产品名称
    人M-CSF ELISA试剂盒
    参阅全部 M-CSF 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 5 pg/ml
  • 范围
    2.78 pg/ml - 2000 pg/ml
  • 复检

    83 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 81.45 70% - 94%
    Serum 86.78 73% - 96%
    Plasma 84.67 71% - 95%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s MCSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCSF in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MCSF coated on a 96-well plate. Standards and samples are pipetted into the wells and MCSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MCSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MCSF 2 vials
    MCSF Microplate (12 x 8 wells) 1 unit
    Recombinant Human MCSF Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • 翻译后修饰
    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • 细胞定位
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 别名
    • Colony stimulating factor 1
    • Colony stimulating factor 1 (macrophage)
    • Colony stimulating factor macrophage specific
    • CSF 1
    • CSF-1
    • CSF1
    • CSF1_HUMAN
    • Csfm
    • Lanimostim
    • M CSF
    • M-CSF
    • Macrophage colony stimulating factor
    • Macrophage Colony Stimulating Factor 1
    • MCSF
    • MGC31930
    • Processed macrophage colony-stimulating factor 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab100590.

  • Representative Standard Curve using ab100590.

实验方案

文献

ab100590 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us.

We have 1 kit for human CSF-1 (we call it MCSF):
ab100590 (http://www.abcam.com/MCSF-Human-ELISA-Kit-1-x-96-Well-Plate-ab100590.html)

The price is USD$470.00 for 1x 96 tests.

I hope thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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