人Lysozyme ELISA试剂盒(LZM) (ab108880)
Key features and details
- Sensitivity: 39 pg/ml
- Range: 0.156 ng/ml - 5 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Other biological fluids, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Lysozyme ELISA试剂盒(LZM)
参阅全部 Lysozyme 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5% 批次间 样品 n Mean SD CV% Overall 10.4% -
样品类型
Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 39 pg/ml -
范围
0.156 ng/ml - 5 ng/ml -
回收率
= 98 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human Lysozyme (LZM) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Lysozyme in plasma, serum, urine, saliva, and CSF.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lysozyme specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lysozyme captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Lysozyme Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Lysozyme Microplate (12 x 8 well strips) 1 unit Lysozyme Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
疾病相关
Amyloidosis 8 -
序列相似性
Belongs to the glycosyl hydrolase 22 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
see all -
数据库链接
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108880 被引用在 7 文献中.
- Papademas P et al. Investigation of donkey milk bacterial diversity by 16S rDNA high-throughput sequencing on a Cyprus donkey farm. J Dairy Sci 104:167-178 (2021). PubMed: 33162091
- Rana T et al. Linking bacterial enterotoxins and alpha defensin 5 expansion in the Crohn's colitis: A new insight into the etiopathogenetic and differentiation triggers driving colonic inflammatory bowel disease. PLoS One 16:e0246393 (2021). PubMed: 33690604
- Furtado GE et al. Combined Chair-Based Exercises Improve Functional Fitness, Mental Well-Being, Salivary Steroid Balance, and Anti-microbial Activity in Pre-frail Older Women. Front Psychol 12:564490 (2021). PubMed: 33841224
- Makridakis M et al. Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma. Sci Rep 10:4815 (2020). PubMed: 32179759
- Mao Y et al. Processed human amniotic fluid retains its antibacterial activity. J Transl Med 17:68 (2019). PubMed: 30823930
- Palladino END et al. 2-Chlorofatty acids: lipid mediators of neutrophil extracellular trap formation. J Lipid Res 59:1424-1432 (2018). PubMed: 29739865
- Glorieux G et al. New insights in molecular mechanisms involved in chronic kidney disease using high-resolution plasma proteome analysis. Nephrol Dial Transplant 30:1842-52 (2015). Sandwich ELISA ; Human . PubMed: 26160894