人Lysozyme ELISA试剂盒(LZM) (ab108877)

概述

  • 产品名称人Lysozyme ELISA试剂盒(LZM)
    参阅全部 Lysozyme 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.5%
    批次间
    样品 n Mean SD CV%
    Overall 8.7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Milk
  • 检测类型Competitive
  • 灵敏度
    = 0.09 µg/ml
  • 范围
    0.093 µg/ml - 6 µg/ml
  • 复检

    = 98 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.

    A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • 疾病相关Amyloidosis 8
  • 序列相似性Belongs to the glycosyl hydrolase 22 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • 1
    • 1 4 beta N acetylmuramidase C
    • 4-beta-N-acetylmuramidase C
    • EC 3.2.1.17
    • LYSC_HUMAN
    • Lysosyme
    • Lysozyme (renal amyloidosis)
    • Lysozyme C
    • Lysozyme C precursor
    • LYZ
    • LZM
    • Renal amyloidosis
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human Lysozyme ELISA Kit (LZM) 图像

  • Representative Standard Curve using ab108877.

实验方案

Human Lysozyme ELISA Kit (LZM) (ab108877)参考文献

ab108877 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.
Unfortunately, we do not have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow available in our catalog.
I would like to recommend checking the Biocompare website ...

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Thank you for contacting us.

As suggested on the datasheet, I recommend diluting the samples 1:200 in Diluent. If necessary, further dilute samples within the range of 1:20 to 1:2000 into Diluent.

I hope this information is helpful...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"