概述

  • 产品名称人Lymphotactin ELISA试剂盒
    参阅全部 Lymphotactin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 150 pg/ml
  • 范围
    0.069 ng/ml - 50 ng/ml
  • 复检

    83 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 81.23 75% - 101%
    Serum 97.65 86% - 114%
    Plasma 72.19 68% - 109%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Lymphotactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lymphotactin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Lymphotactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Lymphotactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lymphotactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lymphotactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
  • 组织特异性Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
  • 序列相似性Belongs to the intercrine gamma family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • ATAC
    • C motif chemokine 1
    • Chemokine (C motif) ligand 1
    • Cytokine SCM-1
    • Cytokine SCM1
    • LPTN
    • LTN
    • Lymphotactin
    • Lymphotaxin
    • SCM 1
    • SCM 1 alpha
    • SCM-1-alpha
    • SCM1
    • SCM1 alpha
    • SCYC1
    • Small inducible cytokine C1
    • Small inducible cytokine subfamily C member 1
    • Small-inducible cytokine C1
    • XC chemokine ligand 1
    • Xcl1
    • XCL1_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Lymphotactin ELISA Kit 图像

  • Representative standard curve using ab100584

  • Representative standard curve using ab100584

实验方案

Human Lymphotactin ELISA Kit (ab100584)参考文献

ab100584 has not yet been referenced specifically in any publications.

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