人liver组织裂解液- total protein (ab29889)

概述

  • 产品名称人liver组织裂解液- total protein
    参阅全部 Liver 细胞裂解液
  • 描述
    人liver总蛋白裂解液
  • 常规说明


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • 存储溶液Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • 裂解物说明Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab29889 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    Human liver tissue lysate - total protein 图像

    • All lanes : Anti-Selenium Binding Protein 1 antibody (ab90135)

      Lane 1 : Human liver tissue lysate - total protein (ab29889)
      Lane 2 : Human colon tissue lysate - total protein (ab30051)
      Lane 3 : Human spleen tissue lysate - total protein (ab29699)
      Lane 4 : Human placenta tissue lysate - total protein (ab29745)

      Lysates/proteins at 20 µg per lane.


      Exposure time : 5 seconds
    • All lanes : Anti-GAPDH (expected size 37kDa) at 1/10000 dilution

      Lane 1 : Human liver tissue lysate - total protein (ab29889) at 5 µg
      Lane 2 : Ladder
      Lane 3 : Human ES whole cell lysate at 10 µg

      Secondary
      Donkey Anti-Mouse IgG H&L (HRP) (ab205724) at 1/5000 dilution
      Developed using the ECL technique

      Exposure time : 5 seconds

      Data courtesy of an anonymous collaborator.

      Blocking buffer- 5% BSA /1% OvAlb /PBS (also works with 5% milk powder) for 3 hours.

    Human liver tissue lysate - total protein (ab29889)参考文献

    This product has been referenced in:
    • Jaacks LM  et al. Placental expression of the heme transporter, feline leukemia virus subgroup C receptor, is related to maternal iron status in pregnant adolescents. J Nutr 141:1267-72 (2011). Read more (PubMed: 21593354) »

    See 1 Publication for this product

    Product Wall

    Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

    We have 5 liver lysates that are from different donors:

    Ab44563-

    http://www.abcam.com/Liver-Human-Tissue-Lysate-adult-n...

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    Thank you for calling Abcam today.

    I am sorry about the issues that you have been having with ab13868 in western blot. AS we discussed today, I am sending you a vial of human liver lysate to use as a positive control (although I am sending ab2...

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    Thank you for your enquiry

    We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

    I reviewed the literature and found the following protocol...

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    Thank you for your enquiry and your valuable feedback. We will change the shipping conditions for these products as soon as possible. This is to confirm that we will ship again one vial of each of these lysates as free of charge replacements - on d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"