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EMSA, MS labby MS found non-nuclear proteins in this lysatelysate is more contanimated than exptected (e.g. keratin, albumin, transferrin, and other blood/serum proteins)extensive wash steps used?purity/QC control?how are the l...
Asked on Feb 28 2013
The lab let me know the following regarding the procedure of how the nuclear lysates are prepared as well as their QC methods:Our protocol involves resuspension of the tissue in hypotonic buffer and the addition of detergent to release t...
Abcam Scientific Support
回复于 Feb 28 2013
Is this sample from a cell line or from a human liver. If it is from a liver, are there any pathogens that we need to be aware of?
Asked on Nov 16 2011
Thank you for calling Abcam. I am sorry that you did not receive the first email i sent as i took down your email address incorrectly. The lab has told me that ab14836 is from a human healthy donor and that it was not tested for any pathogens. The buff...
回复于 Nov 16 2011
Asked on Nov 08 2011
Thank you for contacting Abcam. The lab have told me that ab14836 is from a human healthy donor and that it was not tested for any pathogens. If there is anything else I can help you with, please let me know.
回复于 Nov 08 2011
Is there a protocol for using this lysate in an EMSA assay? Any suggestions would be great.
Asked on Oct 31 2011
Thank you for contacting us. This extract has been tested using classical radioactive EMSA. Unfortunately, we do not have a specific protocol available for this application. I am sorry I could not be more helpful, please let me know if you have any ...
回复于 Oct 31 2011
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