人Lipoprotein A ELISA试剂盒(ab108878)

概述

  • 产品名称人Lipoprotein A ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.6%
    批次间
    样品 n Mean SD CV%
    Overall 9.5%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.7 ng/ml
  • 范围
    0.78 ng/ml - 50 ng/ml
  • 复检

    = 98 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    ���反应: Human
  • 产品概述

    Abcam’s Lipoprotein A Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lipoprotein A concentrations in plasma, serum, urine, milk, cerebrospinal fluid(CSF) and cell culture supernatants.


    A Lipoprotein A specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lipoprotein A specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lipoprotein A captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.
  • 序列相似性Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 38 kringle domains.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰N- and O-glycosylated. The N-glycans are complex biantennary structures present in either a mono- or disialylated state. The O-glycans are mostly (80%) represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated and non-sialylated O-glycans also detected.
  • Information by UniProt
  • 别名
    • Apo(a)
    • APOA_HUMAN
    • Apolipoprotein(a)
    • Lp(a)
    • LPA
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Lipoprotein A ELISA Kit 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Lipoprotein A measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum, plasma and milk samples diluted 4000-32000 fold. Human urine and saliva diluted 2-8 fold. Mouse and rat samples diluted 10-100 fold. Cell supernatants diluted 1-10 fold.

实验方案

Human Lipoprotein A ELISA Kit (ab108878)参考文献

ab108878 has not yet been referenced specifically in any publications.

Product Wall



I can confirm that the antibody used in the kit are polyclonal. I am sorry that the immunogen is confidential in this case.

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. We have tested ab108878 batch: GR57178 and...

Read More

Thank you for contacting us. The information regarding the sample and standard dilutions is included under Point 6 of the Protocol “Preparation of Reagents” (Page 7). As you mention, for plasma samples, the recommended dilution is 1/8000. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"