人Lipocalin 2 ELISA试剂盒(NGAL) (ab113326)

概述

  • 产品名称
    人Lipocalin 2 ELISA试剂盒(NGAL)
    参阅全部 Lipocalin-2 / NGAL 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    4.1 pg/ml - 1000 pg/ml
  • 复检

    96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 75.47 69% - 88%
    Serum 103.9 94% - 112%
    Plasma 109.3 96% - 130%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Lipocalin 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lipocalin 2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Lipocalin 2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Lipocalin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lipocalin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab113326

实验方案

文献

ab113326 has not yet been referenced specifically in any publications.

客户评价及客户问答


The shelf life of each of our kits is indicated in the individual protocol booklet.

ab99995 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80&d...

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Thank you for contacting us.
It is very important for this assay to dilute samples so that the measurements are in the range of the standard curve.
The datasheet indicates a range from 4.1 pg/ml - 1000 pg/ml (= 0.041- 1 ng/ml) for the NGAL kit...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these kits.
The details you have kindly provided will enable us to investigate this case for...

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I am sorry for the delay in getting back to you. I now have further information to share with you in regards to the Lipocalin-2 / NGAL Human ELISA Kit (ab113326).

I can confirm that we would recommend initially using the kit with serum or plas...

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I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Li...

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To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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