人Lipocalin 2 ELISA试剂盒(NGAL) (ab113326)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Lipocalin 2 ELISA试剂盒(NGAL)
参阅全部 Lipocalin-2 / NGAL 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 4 pg/ml -
范围
4.1 pg/ml - 1000 pg/ml -
回收率
96 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 75.47 69% - 88% Serum 103.9 94% - 112% Plasma 109.3 96% - 130% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Lipocalin 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lipocalin 2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Lipocalin 2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Lipocalin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lipocalin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human Lipocalin 2 2 vials Lipocalin 2 Microplate (12 x 8 wells) 1 unit Recombinant Human Lipocalin 2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
组织特异性
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
细胞定位
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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别名
- 24p3
- 25 kDa alpha 2 microglobulin related subunit of MMP9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
see all -
数据库链接
- Entrez Gene: 3934 Human
- Omim: 600181 Human
- SwissProt: P80188 Human
- Unigene: 204238 Human
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab113326 被引用在 4 文献中.
- Huang H et al. Derivation and validation of urinary TIMP-1 for the prediction of acute kidney injury and mortality in critically ill children. J Transl Med 20:102 (2022). PubMed: 35197070
- Pei Y et al. Serum cystatin C, kidney injury molecule-1, neutrophil gelatinase-associated lipocalin, klotho and fibroblast growth factor-23 in the early prediction of acute kidney injury associated with sepsis in a Chinese emergency cohort study. Eur J Med Res 27:39 (2022). PubMed: 35272698
- Abas AM et al. Diagnostic and Prognostic Role of Serum Omentin and NGAL Levels in Egyptian Breast Cancer Patients. Int J Breast Cancer 2022:5971981 (2022). PubMed: 36160364
- Darawshi S et al. Biomarker evidence for distal tubular damage but cortical sparing in hospitalized diabetic patients with acute kidney injury (AKI) while on SGLT2 inhibitors. Ren Fail 42:836-844 (2020). PubMed: 32787602