概述

  • 产品名称人LIMP II ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    10.24 pg/ml - 2500 pg/ml
  • 复检

    86 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.06 74% - 107%
    Serum 83 74% - 92%
    Plasma 79.65 71% - 86%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s LIMP II Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIMP-II in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human LIMP II coated on a 96-well plate. Standards and samples are pipetted into the wells and LIMP II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIMP II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIMP II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Act as a lysosomal receptor for glucosylceramidase (GBA) targeting.
  • 疾病相关Defects in SCARB2 are the cause of progressive myoclonic epilepsy type 4 with or without renal failure (EPM4) [MIM:254900]. An autosomal recessive progressive myoclonic epilepsy associated with renal failure in some cases. Cognitive function is preserved. Myoclonus is a brief, involuntary twitching of a muscle or a group of muscles. Cognitive function is preserved.
    Note=Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
  • 序列相似性Belongs to the CD36 family.
  • 细胞定位Lysosome membrane.
  • Information by UniProt
  • 别名
    • 85 kDa lysosomal membrane sialoglycoprotein
    • CD36
    • CD36 antigen-like 2
    • LGP85
    • LIMP II
    • Lysosome membrane protein 2
    • Lysosome membrane protein II
    • Scarb2
    • Scavenger receptor class B member 2
    • SCRB2_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human LIMP II ELISA Kit 图像

  • Representative Standard Curve using ab113336

实验方案

Human LIMP II ELISA Kit (ab113336)参考文献

ab113336 has not yet been referenced specifically in any publications.

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